renal carcinoma cell lines  (ATCC)

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 mRNA and protein expression was significantly upregulated in ccRCC tissues and cell lines and its relationship to clinicopathological parameters (A and B) The protein and mRNA levels of KIAA1429 in ccRCC and normal kidney tissues. (C and D) KIAA1429 mRNA and protein expression in ccRCC cell lines and normal epithelium cell of renal tubule (HK2) cells. (E and F) The expression level of KIAA1429 in ccRCC tissue and surrounding normal renal tissue was detected using IHC. Scale bars, 100 μm. (G) The expression level of KIAA1429 in patients with ccRCC with different clinical stages and Fuhrman’s grade. (H) Effect of KIAA1429 expression on the overall survival of patients with ccRCC. T, tumor; N, normal. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: The human normal renal tubular epithelial cells (HK2) and renal carcinoma cell lines (786-O, ACHN, Caki-1) were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Two Tailed Test

Journal: PLOS Computational Biology

Article Title: CASER: A semi-supervised model with multi-omics data integration prioritizes cancer-associated epigenetic regulator genes

doi: 10.1371/journal.pcbi.1014253

Figure Lengend Snippet: (A) Six predicted cER genes were investigated in SK-mel-2 cell line ( n = 4). (B) Six predicted cER genes were investigated in Caki-1 cell line ( n = 4). Statistical analysis was performed between si-NC and si-Genes. P -values are calculated by One-way analysis of variance followed by Dunnett’s corrections and indicated by star symbols, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05.

Article Snippet: The human melanoma cell line SK-mel-2, clear cell renal cell carcinoma cell line Caki-1, breast cancer cell line MDA-MB-231, and prostate cancer cell line LNCaP, along with their corresponding complete median, were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Exploratory Multi-Level Analysis of the HIF Axis in Clear-Cell Renal Cell Carcinoma and Evaluation of GN44028 as an Experimental HIF Pathway-Modulating Compound

doi: 10.3390/ijms27083505

Figure Lengend Snippet: Cytotoxicity of HIF inhibitors and sunitinib in renal cell lines: ( A ) 786-O, ( B ) Caki-1, and ( C ) HK-2. Dose–response curves (SRB, 72 h of incubation). Data are presented as mean ± SD from independent biological experiments, each measured in technical replicates ( n = 6). Curves were fitted using four-parameter logistic regression.

Article Snippet: 786-O (cRL1932TM), Caki-1 (McCoy) human renal cell carcinoma lines, and HK-2 (cRL2190TM) human proximal kidney tubule-derived cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Incubation

Journal: International Journal of Molecular Sciences

Article Title: Exploratory Multi-Level Analysis of the HIF Axis in Clear-Cell Renal Cell Carcinoma and Evaluation of GN44028 as an Experimental HIF Pathway-Modulating Compound

doi: 10.3390/ijms27083505

Figure Lengend Snippet: Cell cycle analysis by propidium iodide staining and flow cytometry in 786-O ( A ), Caki-1 ( B ), and HK-2 ( C ) cells treated with KC7F2, FM19G11, GN44028, and sunitinib at IC 50 concentrations for 24 h. Bar graphs show mean ± SD from independent biological experiments, each measured in technical replicates ( n = 3–19). GN44028 was associated with an increased sub-G1 fraction in ccRCC cell lines and G2/M arrest in HK-2 cells. Sunitinib induces G0/G1 arrest in 786-O cells. **** p < 0.0001, * p < 0.05 vs. DMSO (ANOVA with Dunnett’s test).

Article Snippet: 786-O (cRL1932TM), Caki-1 (McCoy) human renal cell carcinoma lines, and HK-2 (cRL2190TM) human proximal kidney tubule-derived cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Cell Cycle Assay, Staining, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Exploratory Multi-Level Analysis of the HIF Axis in Clear-Cell Renal Cell Carcinoma and Evaluation of GN44028 as an Experimental HIF Pathway-Modulating Compound

doi: 10.3390/ijms27083505

Figure Lengend Snippet: Representative images from the wound closure assay. ( A ) 786-O cells were imaged at 0, 10, and 20 h. ( B ) Caki-1 cells were imaged at 0, 24, and 48 h. The compounds are arranged from top to bottom: DMSO, KC7F2, GN44028, FM19G11, and sunitinib. Scale bar: 100 µm. Magnification 400×.

Article Snippet: 786-O (cRL1932TM), Caki-1 (McCoy) human renal cell carcinoma lines, and HK-2 (cRL2190TM) human proximal kidney tubule-derived cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Wound Closure Assay

Journal: International Journal of Molecular Sciences

Article Title: Exploratory Multi-Level Analysis of the HIF Axis in Clear-Cell Renal Cell Carcinoma and Evaluation of GN44028 as an Experimental HIF Pathway-Modulating Compound

doi: 10.3390/ijms27083505

Figure Lengend Snippet: Quantitative analysis of wound closure dynamics. ( A ) 786-O cells (0–20 h). ( B ) Caki-1 cells (0–48 h). Y-axis: Wound closure in culture [%]. The compounds are arranged from top to bottom: DMSO (yellow), KC7F2 (blue), GN44028 (green), FM19G11 (red), and sunitinib (black). Data are presented as mean ± SD from independent biological experiments, each measured in technical replicates ( n = 18–23). **** p < 0.0001 vs. DMSO (one-way ANOVA + Dunnett’s test).

Article Snippet: 786-O (cRL1932TM), Caki-1 (McCoy) human renal cell carcinoma lines, and HK-2 (cRL2190TM) human proximal kidney tubule-derived cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Exploratory Multi-Level Analysis of the HIF Axis in Clear-Cell Renal Cell Carcinoma and Evaluation of GN44028 as an Experimental HIF Pathway-Modulating Compound

doi: 10.3390/ijms27083505

Figure Lengend Snippet: Heatmap of transcriptional responses of hypoxia- and HIF-regulated genes in renal cell carcinoma cell lines following pharmacological inhibition of the HIF pathway. The heatmap shows log 2 (fold change) values of mRNA expression relative to vehicle-treated controls (DMSO) for 786-O and Caki-1 cells after 72 h of treatment with KC7F2, GN44028, FM19G11, or sunitinib (SUN) at IC 50 concentrations. Normal proximal tubule cells (HK-2) were excluded from visualisation. All qPCR data are presented as log 2 fold change relative to the vehicle-treated controls (DMSO). Red, green, and white colours indicate downregulation, upregulation, and no change in expression, respectively. The colour scale represents log 2 FC values ranging from −5 to +5. Genes were hierarchically clustered (average linkage, Euclidean distance), and treatment conditions were ordered manually. Each value represents the mean of two independent biological experiments, each with three technical replicates.

Article Snippet: 786-O (cRL1932TM), Caki-1 (McCoy) human renal cell carcinoma lines, and HK-2 (cRL2190TM) human proximal kidney tubule-derived cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Inhibition, Expressing

Journal: The Journal of Clinical Investigation

Article Title: Genome-wide CRISPR screen identifies a cytokine-enhancer circuit driving HIF-2 α activation in renal cancer

doi: 10.1172/JCI201639

Figure Lengend Snippet: ( A ) Top genes identified for negative regulation of HIF-2α activity versus their log 10 RRA scores in the genome-wide loss-of-function screen. Red circles are regulators reported previously. ( B ) Distribution of sgRNA LFC comparing the mCherry-enriched group with the presorted group (negative regulators). Red bars represent sgRNAs for those genes. ( C ) Representative images of mCherry fluorescence in 786-O HIF-2α reporter cells transduced with 2 individual sgRNAs targeting SOCS3 . Scale bars: 9μM. ( D ) FACS-based analysis of HIF-2α activity in 786-O HIF-2α reporter cells following SOCS3 KO using 2 independent sgRNAs. ( E and F ) Immunoblot analysis of the kidney epithelial cell lines HKC ( E ) and HK-2 ( F ) transduced with sgRNAs targeting SOCS3 or VHL using the indicated antibodies. ( G – K ) Immunoblot analysis of the ccRCC cell lines 786-O ( G ), A498 ( H ), UMRC6 ( I ), Caki-1 ( J ), and PDX XP258 cells ( K ) transduced with sgRNAs targeting SOCS3 or EPAS1 using the indicated antibodies. ( L – S ) RT-qPCR quantification of HIF2A mRNA levels of the indicated cell lines transduced with sgCtrl or sgRNAs targeting SOCS3 . * P < 0.05, ** P < 0.01 *** P < 0.001, and **** P < 0.0001, by 1-way ANOVA followed by Tukey’s multiple-comparison test. Data show the mean ± SEM.

Article Snippet: 786-O, A498, 293 T, HKC, U2OS, and Caki-1 cells were purchased from the American Type Culture Collection (ATCC); UMRC2 and UMRC6 cells were purchased from MilliporeSigma; and HK-2 cells were acquired from Peter Ly’s laboratory at UTSW.

Techniques: Activity Assay, Genome Wide, Fluorescence, Transduction, Western Blot, Quantitative RT-PCR, Comparison